2I5N

1.96 A X-ray structure of photosynthetic reaction center from Rhodopseudomonas viridis:Crystals grown by microfluidic technique

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Detector technology	CCD
Collection date	2006-07-17
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 43 21 2
Unit cell lengths	220.400, 220.400, 113.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.960
R-factor	0.173
Rwork	0.172
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dxr
RMSD bond length	0.008
RMSD bond angle	1.329
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.960	1.960
Rmerge	0.082	0.480
Number of reflections	189189
<I/σ(I)>	17.5	2.3
Completeness [%]	94.5	67.4
Redundancy	5.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				THE CRYSTALS WERE GROWN BY MICROFLUIDIC TECHNIQUE USING CRYSTALLIZATION BUFFER: 1.6 - 1.8 M (NH4)2SO4 IN TRIS PH 7.8. THE ADDITIVES WERE HEPTANETRIOL (3%, W/V) AND 2% TRIETHYL AMONIUM PHOSPHATE. THE DETERGENT WAS LAURYL DIMETHYLAMINE-N-OXIDE (LDA). THE PROTEIN COMPLEX WAS IN SODIUM PHOSPHATE BUFFER PH 6.0 AND 0.08% LDA.