2I5F
Crystal structure of the C-terminal PH domain of pleckstrin in complex with D-myo-Ins(1,2,3,5,6)P5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Detector technology | CCD |
Collection date | 2006-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.150, 47.700, 64.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.700 - 1.350 |
R-factor | 0.17902 |
Rwork | 0.179 |
R-free | 0.20833 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.034 |
RMSD bond angle | 3.270 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.030 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.044 | 0.454 |
Number of reflections | 21808 | |
<I/σ(I)> | 25.1 | 3.9 |
Completeness [%] | 97.7 | 95.9 |
Redundancy | 12.21 | 7.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297.15 | 0.1 M Bis-Tris pH 6.5 28% PEG 2000 MME , VAPOR DIFFUSION, HANGING DROP, temperature 297.15K |