2I4O
Rhodopseudomonas palustris prolyl-tRNA synthetase in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.200, 211.210, 148.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.400 |
| R-factor | 0.20051 |
| Rwork | 0.197 |
| R-free | 0.26104 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | native R. palustris Prolyl-tRNA synthetase |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.566 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 65656 | |
| Completeness [%] | 93.6 | 93.7 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M hepes (pH 7.0), 8-10 % PEG 8K, 5-10 % ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
