2I46

Crystal structure of human TPP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9795
Spacegroup name	I 41 2 2
Unit cell lengths	117.246, 117.246, 171.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
Rwork	0.213
R-free	0.25090
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.281
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.116	0.385
Number of reflections	16372
<I/σ(I)>	2.7	4.3
Completeness [%]	92.4	81.7
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	3.5 M Sodium formate, 0.1 M sodium citrate, pH 5.6, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 289K