2I3T
Bub3 complex with Mad3 (BubR1) GLEBS motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-10-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.18070 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.352, 173.335, 90.019 |
| Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.242 |
| Rwork | 0.238 |
| R-free | 0.26730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u4c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.367 | |
| Number of reflections | 40970 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 95.2 | 76.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 17% PEG 4K 10% isopropanol 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
