2I3R
Engineered catalytic domain of protein tyrosine phosphatase HPTPbeta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.856, 71.643, 70.531 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 31.900 - 1.850 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h02 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.900 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.032 | 0.162 |
| Number of reflections | 51856 | |
| <I/σ(I)> | 19.6 | 6.09 |
| Completeness [%] | 98.5 | 99.9 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 21% PEG 8000, 220 mM MgCl2, 1% BME, 0.1% BOG, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
