2I39
Crystal structure of Vaccinia virus N1L protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.12 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.640, 109.990, 69.580 |
| Unit cell angles | 90.00, 110.92, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| Rwork | 0.202 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 47333 | |
| <I/σ(I)> | 12.7 | 2.1 |
| Completeness [%] | 95.7 | 79.3 |
| Redundancy | 2.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 10% PEG 4K, 0.1 M Tris HCl, 0.1 M sodium-potassium tartrate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
