2I33
The structure of the Class C acid phosphatase from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 140 |
| Detector technology | CCD |
| Collection date | 2005-10-14 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.2037 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.231, 89.909, 104.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.120 - 1.570 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.20700 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.318 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.120 | 1.660 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 66821 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1 M HEPES pH 7.0 30% v/v Jeffamine ED-2001 reagent, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
