2I1S
Crystal Structure of Protein of Unknown Function MM3350 from Methanosarcina mazei Go1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97936, 0.97953 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.421, 90.577, 107.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.19456 |
| Rwork | 0.192 |
| R-free | 0.24284 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.613 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | 0.416 |
| Number of reflections | 26070 | |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 93.5 | 59.7 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Sodium citrate 20% isopropanol 20% PEG 4000 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
