2I0W
Crystal structure analysis of NP24-I, a thaumatin-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 61.010, 61.010, 62.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.500 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pcv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.114 | 0.337 |
| Number of reflections | 8574 | |
| <I/σ(I)> | 3.9 | 1.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.06 | 4.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 0.1M lithium chloride, 20% PEG 3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
