2I0I
X-ray crystal structure of Sap97 PDZ3 bound to the C-terminal peptide of HPV18 E6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-05-07 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 94.167, 61.919, 57.142 |
Unit cell angles | 90.00, 123.33, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.800 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 6381 |
Completeness [%] | 93.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | PEG4000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, temperature 291K |