2I0F
Lumazine synthase RibH1 from Brucella abortus (Gene BruAb1_0785, Swiss-Prot entry Q57DY1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.438 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.101, 68.521, 90.649 |
| Unit cell angles | 90.00, 108.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.220 |
| R-factor | 0.2449 |
| Rwork | 0.242 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f59 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.149 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.041 | 0.282 |
| Number of reflections | 33602 | |
| <I/σ(I)> | 12.3 | 2.6 |
| Completeness [%] | 94.8 | 88.7 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | 12% PEG 4000, 0.1M HEPES, 0.2M CALCIUM CHLORIDE, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
