2I08
Solvation effect in conformational changes of EF-hand proteins: X-ray structure of Ca2+-saturated double mutant Q41L-K75I of N-domain of calmodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 38.400, 38.400, 81.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.730 - 2.000 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cll |
RMSD bond length | 0.014 |
RMSD bond angle | 1.600 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.700 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 5553 | |
Completeness [%] | 99.3 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 36% (v/v) PEG 400, 100 mM HEPES, 200 mM CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |