2HZS

Structure of the Mediator head submodule Med8C/18/20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	75.365, 115.762, 129.159
Unit cell angles	90.00, 98.49, 90.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.236
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hzm
RMSD bond length	0.008
RMSD bond angle	1.261
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	61548
<I/σ(I)>	19.2	2.1
Completeness [%]	99.9	99.1
Redundancy	6.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	100 mM sodium acetate, 0.6 M sodium fluoride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K