2HZH
Crystal structure of laccase from Coriolus zonatus at 2.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 168.930, 168.930, 69.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 145.860 - 2.600 |
| R-factor | 0.21272 |
| Rwork | 0.211 |
| R-free | 0.23792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 145.860 | 2.620 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 35011 | |
| Completeness [%] | 1.0 | 95.2 |
| Redundancy | 5.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | The crystallizing solution (volume 6 ml) contained the protein at a concentration of 8 mg/ml in 50 mM sodium citrate, 0.1 M ammonium sulfate, and 12.5% (w/v) PEG 4000 in 0.05M sodium acetate buffer at pH 4.6., pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
