2HZB

X-Ray Crystal Structure of Protein BH3568 from Bacillus halodurans. Northeast Structural Genomics Consortium BhR60.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793, 0.9796, 0.9681
Spacegroup name	P 21 21 21
Unit cell lengths	51.725, 142.012, 229.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.800
R-factor	0.185
Rwork	0.185
R-free	0.23900
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.300
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.092	0.399
Number of reflections	71260
<I/σ(I)>	13.6	3
Completeness [%]	98.5	95.1
Redundancy		8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	277	2.4M Sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.00