2HYZ
Crystal structure of an 8 repeat consensus TPR superhelix (orthorombic crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.50 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.156, 67.695, 70.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.259 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.06) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.101 | 0.054 | 0.208 |
| Number of reflections | 14975 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 99.9 | 99.5 | 99.5 |
| Redundancy | 3.4 | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | 100 mM Acetate, 25% MPD, 50 mM SmCl3, pH 5.0, VAPOR DIFFUSION, temperature 298K |
