2HYZ

Crystal structure of an 8 repeat consensus TPR superhelix (orthorombic crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1.50
Spacegroup name	P 21 21 21
Unit cell lengths	36.156, 67.695, 70.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.217
Rwork	0.213
R-free	0.27000
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.259
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.101	0.054	0.208
Number of reflections	14975
<I/σ(I)>	9.2
Completeness [%]	99.9	99.5	99.5
Redundancy	3.4	3.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	298	100 mM Acetate, 25% MPD, 50 mM SmCl3, pH 5.0, VAPOR DIFFUSION, temperature 298K