2HYW

Human Annexin A2 with Calcium bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-10-01
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	60.509, 64.283, 104.158
Unit cell angles	90.00, 91.73, 90.00

Refinement procedure

Resolution	40.000 - 2.100
Rwork	0.214
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1XJL
RMSD bond length	0.005
RMSD bond angle	1.050
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR (2.5)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.150
High resolution limit [Å]	2.100	5.180	2.100
Rmerge	0.067	0.030	0.331
Number of reflections	45423
<I/σ(I)>	12.8
Completeness [%]	96.7	97.9	93.6
Redundancy	3.6	3.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	8.5	290	PEG 8000, pH 8.5, hanging drop, temperature 290K
1	hanging drop	8.5	290	PEG 8000, pH 8.5, hanging drop, temperature 290K