2HYK
The crystal structure of an endo-beta-1,3-glucanase from alkaliphilic Nocardiopsis sp.strain F96
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.589, 71.843, 39.666 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 39.650 - 1.300 |
| R-factor | 0.144 |
| Rwork | 0.143 |
| R-free | 0.16200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.08) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.064 | 0.350 |
| Number of reflections | 47558 | |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 99.3 | 95.7 |
| Redundancy | 6.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl pH 8.0, 1.3-1.4M Ammonium sulfate, 1% Ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
