2HY7

Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-27
Detector	ADSC QUANTUM 210
Spacegroup name	P 65 2 2
Unit cell lengths	123.629, 123.629, 174.307
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	107.210 - 1.900
R-factor	0.182
Rwork	0.181
R-free	0.20400
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.277
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.211	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.069	0.344
Number of reflections	62401
<I/σ(I)>	9.9	2.2
Completeness [%]	100.0	100
Redundancy	14.1	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.2	293	0.1 M Tris-HCl, 35% (w/v) PEG 3350, 0.2 M Li2SO4, 0.1 M CsCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.20
1		8.2	293	0.1 M Tris-HCl, 35% (w/v) PEG 3350, 0.2 M Li2SO4, 0.1 M CsCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.20