2HXS
Crystal Structure of Rab28A GTPase in the Inactive (GDP-3'P-Bound) Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.510, 56.621, 85.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.000 - 1.100 |
R-factor | 0.152 |
Rwork | 0.149 |
R-free | 0.18360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yzk |
RMSD bond length | 0.024 |
RMSD bond angle | 2.184 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.075 | 0.367 |
Number of reflections | 58782 | |
<I/σ(I)> | 24.7 | 4.25 |
Completeness [%] | 98.3 | 96.8 |
Redundancy | 8.3 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 3360, 100 mM NaCl, 100 mM Potassium Acetate, 5% glycerol, 20 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |