2HWW
Structure of PIN domain of human SMG6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.879, 71.216, 181.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.800 - 1.800 |
| R-factor | 0.22417 |
| Rwork | 0.222 |
| R-free | 0.26748 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2HWX |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.840 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 45496 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 291 | Jeffamine 2000 32%, 0.1M Hepes pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
