2HW7

Crystal Structure of Mnk2-D228G in complex with Staurosporine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-08-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.98008
Spacegroup name	P 32 2 1
Unit cell lengths	102.366, 102.366, 76.439
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.710
R-factor	0.2034
Rwork	0.201
R-free	0.24566
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ac3
RMSD bond length	0.010
RMSD bond angle	1.389
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Number of reflections	12862
Completeness [%]	99.7
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	293	Polyacrylic acid, pH 7.6, VAPOR DIFFUSION, temperature 293K