2HW6

Crystal structure of Mnk1 catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-04
Detector	MAR CCD 165 mm
Wavelength(s)	0.98008
Spacegroup name	P 43 21 2
Unit cell lengths	93.468, 93.468, 175.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.20911
Rwork	0.207
R-free	0.25701
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ac3
RMSD bond length	0.010
RMSD bond angle	1.174
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500
Number of reflections	18256
Completeness [%]	92.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	293	20% PEG3350, 0.2M Ammonium sulfate, pH 5.6, VAPOR DIFFUSION, temperature 293K