Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 279 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.300, 57.720, 82.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.149 |
| Rwork | 0.149 |
| R-free | 0.19600 * |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.900 * |
| Data reduction software | MOSFLM |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | |
| High resolution limit [Å] | 1.800 * | 1.800 * |
| Rmerge | 0.048 * | 0.149 * |
| Total number of observations | 107989 * | |
| Number of reflections | 23188 | |
| Completeness [%] | 96.8 | 80.8 * |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | Rozeboom, H.J., (1990) J.Mol.Biol., 212, 441. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 35-65 (%sat) | |
| 2 | 1 | reservoir | sodium chloride | 1.7-3.4 (M) | precipitant |
