2HVG
Crystal Structure of Adenylosuccinate Lyase from Plasmodium Vivax
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.419, 177.599, 68.613 |
| Unit cell angles | 90.00, 99.16, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.20919 |
| Rwork | 0.207 |
| R-free | 0.25078 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 | 0.229 |
| Number of reflections | 62489 | |
| <I/σ(I)> | 10.4 | 6.03 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 1.7 M NH42SO4, 0.1 M Na Citrate, 0.2 M K/Na Tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
