2HVD
Human nucleoside diphosphate kinase A complexed with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.973 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.260, 114.260, 89.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.650 - 2.150 |
R-factor | 0.24911 |
Rwork | 0.247 |
R-free | 0.29374 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ucn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.299 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.650 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 30827 | |
<I/σ(I)> | 10.2 | 3.1 |
Completeness [%] | 97.2 | 99 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 2.4 M AMMONIUM SULPHATE,10 MM ADP, 20 MM MGCL2, 4 MM DTT, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |