2HV9

Encephalitozoon cuniculi mRNA Cap (Guanine-N7) Methyltransferase in complex with sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-10-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	63.810, 63.810, 112.124
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.360 - 2.600
R-factor	0.203
Rwork	0.203
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z3c
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.097	0.426
Number of reflections	8580
<I/σ(I)>	12.4	2.6
Completeness [%]	98.1	96.8
Redundancy	4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.25	291	1.2 M SODIUM/POTASSIUM TARTRATE, 0.05 M bis(2-hydroxyethyl)amino-tris(hydroxy-methyl)methane, 0.02 M DTT, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 291K