2HV6

Crystal structure of the phosphotyrosyl phosphatase activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	158.250, 42.790, 75.570
Unit cell angles	90.00, 108.83, 90.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.19
Rwork	0.183
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 2G62
RMSD bond length	0.008
RMSD bond angle	1.370
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	36825
Completeness [%]	95.4	94.6
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	0.2mM Mg Formate, 5% glycerol, 17.5% PEG3350 (w/v), 0.1 M Bistris, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K