2HUV
Crystal structure of PH0725 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-27 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 104.731, 104.731, 137.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.990 - 2.100 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.22700 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1vce |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 | 0.580 |
| Number of reflections | 44978 | |
| <I/σ(I)> | 11.2 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 295 | Sodium Formate, Acetate, pH 5.5, microbatch, temperature 295K |
