2HUB
Structure of Hen Egg-White Lysozyme Determined from crystals grown in pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9737 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.861, 76.861, 35.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.310 - 1.201 |
| R-factor | 0.138 |
| Rwork | 0.137 |
| R-free | 0.17300 |
| Structure solution method | MAD |
| Starting model (for MR) | In-house structure |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.697 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.2) |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.310 | 1.220 |
| High resolution limit [Å] | 1.201 | 1.201 |
| Rmerge | 0.054 | 0.475 |
| Number of reflections | 33587 | |
| <I/σ(I)> | 16.8 | 2.1 |
| Completeness [%] | 98.2 | 74.3 |
| Redundancy | 6.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch, Douglas Instrument robot | 7.5 | 293 | 0.1 M HEPES-Na pH 7.5, 0.8M Potassium Sodium Tartrate tetrahydrate, Microbatch, Douglas Instrument robot, temperature 293K |
