2HU3
Parent Structure of Hen Egg White Lysozyme grown in acidic pH 4.8. Refinement for comparison with crosslinked molecules of lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9737 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.870, 78.870, 36.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.730 - 1.300 |
| R-factor | 0.105 |
| Rwork | 0.103 |
| R-free | 0.14500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | In-house model |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.571 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.2) |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.730 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.057 | 0.538 |
| Number of reflections | 29161 | |
| <I/σ(I)> | 10.5 | 3.92 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 27 | 15.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 0.1M Na Acetate, pH 4.8, 7% NaCl, 25% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
