2HTL
Structure of the Escherichia coli ClC chloride channel Y445F mutant and Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9193 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.525, 98.860, 171.804 |
| Unit cell angles | 90.00, 131.54, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.400 |
| R-factor | 0.26163 |
| Rwork | 0.259 |
| R-free | 0.30212 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.189 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.081 | |
| Number of reflections | 37989 | |
| Completeness [%] | 99.2 | 98.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 39% peg 300, 50mM Tris, 150mM NaKTart, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
