2HTH

Structural basis for ubiquitin recognition by the human EAP45/ESCRT-II GLUE domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.10000, 0.97910
Spacegroup name	P 42 21 2
Unit cell lengths	102.727, 102.727, 54.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.320 - 2.700
R-factor	0.25184
Rwork	0.248
R-free	0.28872
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.681
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.10)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.109	0.080	0.536
Number of reflections	8264
<I/σ(I)>	11
Completeness [%]	97.7	99.6	81.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	10% PEG8000; 10% Ethylene glycol; 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K