2HT0
IHF bound to doubly nicked DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 1995-06-01 |
Detector | FUJI |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.942, 58.944, 181.676 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.810 - 2.000 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.27800 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 33359 | |
Completeness [%] | 95.3 | 83.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1mM protein, 1.5 molar excess DNA, ~22%PEG4000, 50mM KCl, 1mM spermine, 5-15% glycerol, 15mM Cd++, 0.3% NaN3, 50mM Tris-HCl soaked in cryoprotectant with more glycerol, more PEG, and Mg++ replacing Cd++, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
10 | 1 | 2 | KCl | ||
11 | 1 | 2 | glycerol | ||
12 | 1 | 2 | Cd++ | ||
13 | 1 | 2 | NaN3 | ||
14 | 1 | 2 | Tris-HCl | ||
15 | 1 | 2 | H2O | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | spermine | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 1 | Cd++ | ||
6 | 1 | 1 | NaN3 | ||
7 | 1 | 1 | Tris-HCl | ||
8 | 1 | 1 | H2O | ||
9 | 1 | 2 | PEG4000 |