2HS5
Structural Genomics, the crystal structure of a putative transcriptional regulator GntR from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 108.052, 108.052, 107.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.210 - 2.200 |
| R-factor | 0.19673 |
| Rwork | 0.194 |
| R-free | 0.24851 |
| Structure solution method | SAD |
| RMSD bond length | 0.037 |
| RMSD bond angle | 2.599 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.210 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.076 | 0.540 |
| Number of reflections | 16123 | |
| <I/σ(I)> | 31.84 | 2.44 |
| Completeness [%] | 97.9 | 81.1 |
| Redundancy | 7.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 35% Tacsimate, 0.1M Sodium Acetate, 2% PEG5000 MME, 2mM Cysteine, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
