2HRO
Structure of the full-lenght Enzyme I of the PTS system from Staphylococcus carnosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 173.364, 46.852, 85.297 |
Unit cell angles | 90.00, 101.46, 90.00 |
Refinement procedure
Resolution | 34.860 - 2.500 |
R-factor | 0.2151 |
Rwork | 0.215 |
R-free | 0.26700 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.180 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.860 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 23570 | |
Completeness [%] | 97.4 | 93.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 4000, 0.2 M Li2SO4 and 0.1 M Tris-HCl pH8.5 and 0.2 l of additive solution (solution No 11 of Hampton Crystal Screen, Hampton Research, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 4000, 0.2 M Li2SO4 and 0.1 M Tris-HCl pH8.5 and 0.2 l of additive solution (solution No 11 of Hampton Crystal Screen, Hampton Research, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 4000, 0.2 M Li2SO4 and 0.1 M Tris-HCl pH8.5 and 0.2 l of additive solution (solution No 11 of Hampton Crystal Screen, Hampton Research, VAPOR DIFFUSION, HANGING DROP, temperature 293K |