2HRL
Siglec-7 in complex with GT1b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 46.021, 46.021, 106.474 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.850 |
| R-factor | 0.19678 |
| Rwork | 0.195 |
| R-free | 0.23447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o7v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.486 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.073 | 0.520 |
| Number of reflections | 11626 | |
| <I/σ(I)> | 25.5 | 2.3 |
| Completeness [%] | 98.3 | 98.4 |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 200mM sodium acetate, 100mM sodium cacodylate, 30 % w/v PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
