2HRD

Crystal structure of the uridine phosphorylase from Salmonella typhimurium in complex with thymine and phosphate ion at 1.70A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-15
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	88.200, 123.400, 133.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.16742
Rwork	0.166
R-free	0.18883
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zl2
RMSD bond length	0.006
RMSD bond angle	1.067
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.740
High resolution limit [Å]	1.700	1.700
Number of reflections	159741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			297	25% PEG 400, 0.1M potassium phosphate, 0.2M magnesium chloride, 0.1M cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 297K