2HQY
Crystal Structure of Conserved Protein of Unknown Function from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97936, 0.97953 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.468, 81.879, 138.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.21125 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.649 |
| Number of reflections | 70650 | |
| <I/σ(I)> | 17.1 | 2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 291 | 3M Magnesium Formate 0.1 M BisTris, pH 5.4 10mM CoA, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
