2HQ4
Crystal Structure of ORF 1580 a hypothetical protein from Pyrococcus horikoshii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9790 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.869, 46.392, 94.750 |
Unit cell angles | 90.00, 132.42, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.990 |
R-factor | 0.19654 |
Rwork | 0.195 |
R-free | 0.23184 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.699 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SCA2STRUCTURE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 28050 | |
<I/σ(I)> | 11.6 | |
Completeness [%] | 99.1 | 92.3 |
Redundancy | 6.9 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | HANGING DROP VAPOR DIFFUSION USING 1 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (12 mg/ml) AND RESERVOIR SOLUTION CONTAINinG 0.2M ammonium dihydrogen phosphate, 20% W/V PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |