2HPW
Green fluorescent protein from Clytia gregaria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-01 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.091, 91.446, 110.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.550 |
| R-factor | 0.18271 |
| Rwork | 0.182 |
| R-free | 0.20254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qv1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.407 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 40289 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 86.7 | 20.3 |
| Redundancy | 4.4 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 2 microlitre drops containing protein containing equal volumes of protein solution (9 mg/ml) and precipitate solution (2.0M Ammonium sulfate, 0.1M Citrate), pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
