2HPJ

Crystal structure of the PUB domain of mouse PNGase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-03-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	66.641, 52.042, 34.946
Unit cell angles	90.00, 115.25, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.148
Rwork	0.147
R-free	0.17700
Structure solution method	SIRAS
RMSD bond length	0.014
RMSD bond angle	1.529
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.09)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.055	0.106
Number of reflections	11390
<I/σ(I)>	23.9	6.6
Completeness [%]	95.0	56.8
Redundancy	4.08	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	281	18-22% PEG 5000 MME and 14-18% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K
1	VAPOR DIFFUSION, HANGING DROP	7	281	18-22% PEG 5000 MME and 14-18% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K
1	VAPOR DIFFUSION, HANGING DROP	7	281	18-22% PEG 5000 MME and 14-18% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K