2HPH
High resolution structure of E. coli glucose/galactose binding protein bound with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.260, 36.580, 64.940 |
| Unit cell angles | 90.00, 107.19, 90.00 |
Refinement procedure
| Resolution | 24.570 - 1.330 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.500 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.161 | 0.343 |
| Number of reflections | 62566 | 17054 |
| <I/σ(I)> | 5.22 | 3.18 |
| Completeness [%] | 98.3 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 20% PEG6000, 50mM NaHEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
