2HP9
Crystal Structure of the OXA-10 W154A mutant at pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.330, 94.430, 125.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.170 - 2.500 |
| R-factor | 0.2062 |
| Rwork | 0.203 |
| R-free | 0.26277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2HP5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.170 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.133 | 0.403 |
| Number of reflections | 20280 | |
| <I/σ(I)> | 15 | 5.6 |
| Completeness [%] | 98.9 | 98.4 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 2M Ammonium sulfate, Bicine pH9; the crystal was soaked at pH6 (MES) for 6 days., pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
