2HP5
Crystal Structure of the OXA-10 W154G mutant at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-26 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.110, 125.400, 92.360 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 46.470 - 2.700 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.075 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.470 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.122 | 0.452 |
| Number of reflections | 28824 | |
| <I/σ(I)> | 11 | 3.1 |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | Ammonium Sulfate 2.2 M, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.00 |
