2HNK
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 157.810, 60.331, 138.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| Rwork | 0.213 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.173 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.394 |
| Number of reflections | 58638 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.6 | 99.7 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | 0.1M lithium sulfate monohydrate, 0.1M MES pH 6.5, 9% w/v polyethylene glycol 8000, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
