2HN9

Crystal structure of the uridine phosphorylase from Salmonella typhimurium in complex with thymine and phosphate ion at 2.12A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.8068
Spacegroup name	P 21 21 21
Unit cell lengths	88.800, 123.690, 134.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.120
R-factor	0.19781
Rwork	0.196
R-free	0.22608
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.985
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.173
High resolution limit [Å]	2.120	2.120
Number of reflections	79355
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	VAPOR DIFFUSION, HANGING DROP, temperature 297K