2HN2
Crystal structure of the CorA Mg2+ transporter homologue from T. maritima in complex with divalent cations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9799 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.246, 86.298, 181.527 |
| Unit cell angles | 90.00, 112.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.700 |
| R-factor | 0.2951 |
| Rwork | 0.295 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2BBJ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.058 | 0.461 |
| Number of reflections | 30297 | |
| <I/σ(I)> | 24.9 | |
| Completeness [%] | 91.6 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 20% PEG 400, 0.2 M CaCl2, 0.1 M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
