2HMP

Uncomplexed actin cleaved with protease ECP32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-04
Detector	CUSTOM-MADE
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	63.900, 198.110, 69.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.899
R-factor	0.17947
Rwork	0.178
R-free	0.21487
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j6z
RMSD bond length	0.011
RMSD bond angle	1.277
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.899	1.899
Rmerge	0.073	0.484
Number of reflections	70540
<I/σ(I)>	43.3	7.7
Completeness [%]	99.9	99.7
Redundancy	16.3	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.75	277	1:1 mixture of 5-7.5 mg/ml protein solution containing 0.5 mM ATP and precipitant solution (40 mM SrCl2, 10% ethylene glycol, 13-15% dimethyl polyethylene glycol 5000, 50 mM triethanolamine, 10 mM spermidine, pH 7.75) equilibrated against 0.5 ml precipitant., VAPOR DIFFUSION, HANGING DROP, temperature 277K, VAPOR DIFFUSION, HANGING DROP